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Overview of membrane structure, history
Layers and hydrophilicity, hydrophobicity
Lipid layers
Phospholidids (glycerol plus)
Classes differ
Fatty acids differ
Tri-layered effect under EM
SB = EM prep and image formation
OsO4 is aqueous
Membrane fluidity
Two types of fluidity
Two classical experiments IR dichroism & fluorescent Ab.
SB = antibody ­ immunocytochemistry
Thermotrophic mesomorphism
What controls fluidity?
Membrane proteins
Peripheral vs integral
Types, structures
Pores
Configuration changes
Trafficking
Ordering (e.g., ETS)
Membrane synthesis and assembly (tracing experiments)
SB = isotopes and bionucleonics)
Membrane fusion
Water movement across membranes (y)
Endothelial cells ­ wow!

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