Marcy L. Nelson
Dr. Michael Temkin
SLU Festival of Science 2001 Poster Presentation
Genetic Variation In The Freshwater Mussel Elliptio complanata
From Northern New York
Freshwater mussels are an important group of animals
inhabiting the rivers and lakes of North America. Many native populations
of freshwater mussels in the Adirondack highlands and St. Lawrence lowlands
have been lost or are endangered because of the introduction of the exotic
species Dreissena polymorpha (zebra mussels) and
Dreissena bugensis
(quagga mussels). The loss of populations within the range of a species
may significantly effect the genetic diversity contained within that species.
Here, we examine the genetic variation at two mitochondrial loci of the
freshwater mussel Elliptio complanata. Thirty E. complanata
were collected from thirteen sites in northern New York. After isolating
DNA from individuals, nested PCR protocols were used to amplify a 358 bp
segment of the cytochrome C oxidase subunit I (CO I) gene and a 320 bp
segment of the 16s rRNA gene. To identify how PCR products of each gene
differed, we sequenced each PCR product. We used the DNA sequences of the
CO I and 16s rRNA genes to assess genetic diversity and evaluated the roles
of historical and contemporary processes in determining the genetic variation
within E. complanata populations in northern New York.